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A versatile vector system for multiple gene expression in plants
Authors:Chung Sang-Min  Frankman Ellen L  Tzfira Tzvi
Affiliation:Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, 11794, USA.
Abstract:Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.
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