|
|||||
|
|
A bioluminescent enzyme immunoassay for prostaglandin E2 using Cypridina luciferase |
|
| Authors: | Chun Wu Saki Irie Shozo Yamamoto Yoshihiro Ohmiya |
| Affiliation: | 1. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Osaka, 563‐8577, Japan;2. Department of Food and Nutrition, Faculty of Home Economics, Kyoto Women's University, Kyoto, 605‐8501, Japan;3. Department of Photobiology, Graduate School of Medicine, Hokkaido University, Hokkaido University, Sapporo, 060‐8638, Japan |
| Abstract: | Prostaglandin E2 is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E2 utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E2 amount could be quantified over the concentration ranging from 7.8 to 500 pg/mL. The amount of unlabeled prostaglandin E2 required to displace 50% of the maximal binding of Cypridina luciferase‐labeled prostaglandin E2 (B/B0) was approximately 35 pg/mL. The results show a great potential of Cypridina luciferase as a new labeling enzyme for enzyme‐linked immunosorbent assay. Copyright © 2009 John Wiley & Sons, Ltd. |
| Keywords: | luciferase luciferin immunoassay prostaglandins bioluminescence |