Are nociceptin(1‐13)NH2 and its structural analogue [ORN9]nociceptin(1‐13)NH2 able to affect brain antioxidant status in control and kainic acid‐treated rats? |
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| Authors: | Elina Tzvetanova Almira Pavlova Albena Alexandrova Galina Nenkova Lubomir Petrov Margarita Kirkova Radoslav Girchev Emilia Naydenova |
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| Institution: | 1. Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria;2. Department of Physiology, Medical University, Sofia, Bulgaria;3. Department of Organic Chemistry, University of Chemical Technology and Metallurgy, Sofia, Bulgaria |
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| Abstract: | In‐vivo effects of nociceptin (N/OFQ(1‐13)NH2) on the levels of lipid peroxidation and cell enzyme (superoxide dismutase, glutathione peroxidase and glutathione reductase) and non‐enzyme (glutathione) antioxidants in brain of control and kainic acid‐treated rats were studied. N/OFQ(1‐13)NH2 effects were compared with those of its structural analogue Orn9]N/OFQ(1‐13)NH2. Kainic acid (25 µg, i.c.v) increased the lipid peroxidation (4 and 24 h after kainic acid treatment) and decreased the glutathione level (1 h after kainic acid injection). We failed to find, any changes in antioxidant enzyme activities, independently of the time of kainic acid treatment. At the background of kainic acid‐effects, N/OFQ(1‐13)NH2 and Orn9] N/OFQ(1‐13)NH2, injected 30 min before kainic acid, had no effects on all parameters, tested in brain. In addition, the neuropeptides did not change the antioxidant status in brain of control animals. It might be concluded that N/OFQ(1‐13)NH2 and Orn9]N/OFQ(1‐13)NH2 have neither pro‐ nor anti‐oxidant activity. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | N/OFQ(1‐13)NH2 [Orn9]N/OFQ(1‐13)NH2 kainic acid lipid peroxidation antioxidant enzyme activity |
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