3D culture of osteoblast‐like cells by unidirectional or oscillatory flow for bone tissue engineering |
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| Authors: | Dajiang Du Katsuko S Furukawa Takashi Ushida |
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| Institution: | 1. Department of Mechanical Engineering, Laboratory of Biomedical Engineering, Graduate School of Engineering, University of Tokyo, 2nd Building, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo 113‐8656, Japan;2. Department of Bioengineering, Graduate School of Engineering, University of Tokyo, 2nd Building, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo 113‐8656, Japan;3. telephone: 81‐3‐5841‐6331;4. fax: 81‐3‐5841‐6447;5. Center for NanoBio Integration, University of Tokyo, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo 113‐8656, Japan;6. Division of Biomedical Materials and Systems, Center for Disease Biology and Integrative Medicine, School of Medicine, University of Tokyo, Tokyo, Japan |
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| Abstract: | A medium perfusion system is expected to be beneficial for three‐dimensional (3D) culture of engineered bone, not only by chemotransport enhancement but also by mechanical stimulation. In this study, perfusion systems with either unidirectional or oscillatory medium flow were developed, and the effects of the different flow profiles on 3D culturing of engineered bone were studied. Mouse osteoblast‐like MC 3T3‐E1 cells were 3D‐cultured with porous ceramic scaffolds in vitro for 6 days under static and hydrodynamic conditions with either a unidirectional or oscillatory flow. We found that, in the static culture, the cells proliferated only on the scaffold surfaces. In perfusion culture with the unidirectional flow, the proliferation was significantly higher than in the other groups but was very inhomogeneous, which made the construct unsuitable for transplantation. Only the oscillatory flow allowed osteogenic cells to proliferate uniformly throughout the scaffolds, and also increased the activity of alkaline phosphatase (ALP). These results suggested that oscillatory flow might be better than unidirectional flow for 3D construction of cell‐seeded artificial bone. The oscillatory perfusion system could be a compact, safe, and efficient bioreactor for bone tissue engineering. Biotechnol. Bioeng. 2009;102: 1670–1678. © 2008 Wiley Periodicals, Inc. |
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| Keywords: | tissue engineering perfusion culture in vitro osteogenesis |
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