Selective recognition of thymidine homopolymer (poly T) oligonucleotide with cobalt(II)–4‐[(5‐chloro‐2‐pyridyl)azo]‐1,3‐diaminobenzene complex |
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| Authors: | Feng Ling Guo Yuan Fang Li Cheng Zhi Huang |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, MOE Key Laboratory on Luminescence and Real‐Time Analysis, Southwest University, Chongqing 400 2. 715, People's Republic of China;3. College of Pharmaceutical Sciences, MOE Key Laboratory on Luminescence and Real‐Time Analysis, Southwest University, Chongqing 400 |
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| Abstract: | The interactions of cobalt(II)–4‐[(5‐chloro‐2‐pyridyl)azo]‐1,3‐diaminobenzene (5‐Cl‐PADAB) complex with different kinds of homopolymer oligonucleotides in basic medium were investigated based on the measurements of resonance light scattering, UV–vis, circular dichroism spectra and dark field light‐scattering imaging. Experiments showed that only thymidine homopolymer (poly T) oligonucleotides with the length in the range of poly T6 to poly T18 could interact with the Co(II)–5‐Cl‐PADAB complex in alkaline conditions and cause evident color and spectral change. Thus, the binary complex of Co(II)–5‐Cl‐PADAB could be employed as a visual probe for selectively recognizing the poly T oligonucleotides. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | Poly T oligonucleotides 4‐[(5‐chloro‐2‐pyridyl)azo]‐1,3‐diaminobenzene (5‐Cl‐PADAB) cobalt(II) spectrophotometry recognition |
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