Chiral separation of catechin by capillary electrophoresis using mono‐, di‐, tri‐succinyl‐β‐cyclodextrin as chiral selectors |
| |
| Authors: | Hyunmyung Kim Youngjin Choi Jun Lim Seung‐R Paik Seunho Jung |
| |
| Institution: | 1. Department of Bioscience and Biotechnology and Bio/Molecular Informatics Center, Konkuk University, Seoul 143‐701, South Korea;2. Biochip Research Center, Hoseo University, Asan 336‐795, South Korea;3. School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151‐744, South Korea |
| |
| Abstract: | The chiral separation of (±)‐catechin was investigated by capillary electrophoresis using characterized succinyl‐β‐cyclodextrins (Suc‐β‐CDs) with one to three degree of substitution values. The effects of nature and concentration of Suc‐β‐CDs and running buffer pH on the migration time and resolution of (±)‐catechin are discussed. All three kinds of Suc‐β‐CDs show a clear baseline separation of (±)‐catechin in capillary electrophoresis. Mono‐Suc‐β‐CD effectively separated (±)‐catechin, and additional substituted CDs (di‐ and tri‐Suc‐β‐CD) were capable of chiral separation at a broad pH range. The optimum running conditions were found to be 100 mM borate buffer (pH 9.8) containing 5 mM mono‐Suc‐β‐CD with no methanol organic modifier. Chirality, 2009. © 2009 Wiley‐Liss, Inc. |
| |
| Keywords: | catechin flavanol succinyl‐β ‐cyclodextrin resolution migration time |
|
|
