Determination of trace α‐fetoprotein variant by affinity adsorption solid substrate‐room temperature phosphorimetry |
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| Authors: | Jia‐Ming Liu Zhen‐Bo Liu Xue‐Lin Li Zhi‐Ming Li Xiao‐Mei Huang Feng‐Shan Hong Wei‐Nv Lin Fang Chen |
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| Institution: | 1. Department of Chemistry, Zhangzhou Normal College, Zhangzhou 363000, People's Republic of China;2. The Third Hospital of Xiamen, Xiamen, 316000, People's Republic of China;3. Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, People's Republic of China |
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| Abstract: | The 3.5‐generation dendrimers (3.5G‐D)–porphyrin (P) dual luminescent molecule (3.5G‐D–P) was used to label concanavalin agglutinin (Con A); the product of the reaction is 3.5G‐D–P–Con A. A new method for the determination of trace AFP‐V by affinity adsorption solid substrate–room temperature phosphorimetry (AA‐SS–RTP) was established, based on the room temperature phosphorescence (RTP) property of the product on polyamide membrane (PAM) substrate and the specific affinity adsorption (AA) reaction between 3.5G‐D–P–Con A and α‐fetoprotein variant (AFP‐V), which caused the RTP of the system to be sharply enhanced, the ΔIp was linearly correlated to the content of AFP‐V. The sensitivity of the method was obviously high. It could accurately detect the content of AFP‐V in serum. The results were tallied well with those obtained by the ELISA method. Copyright © 2008 John Wiley & Sons, Ltd. |
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| Keywords: | α ‐fetoprotein variant dendrimers– porphyrin concanavalin agglutinin affinity adsorption solid substrate– room temperature phosphorimetry |
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