In vitro cardiomyogenic differentiation of adipose‐derived stromal cells using transforming growth factor‐β1 |
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| Authors: | So‐Jung Gwak Suk H Bhang Hee S Yang Sang‐Soo Kim Dae‐Hee Lee Soo‐Hong Lee Byung‐Soo Kim |
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| Institution: | 1. Department of Chemical Engineering, Hanyang University, Seoul, Korea;2. Co‐first authors:;3. Department of Bioengineering, Hanyang University, Seoul, Korea;4. Cell and Gene Therapy Research Institute, Pochon CHA University, Seoul, Korea |
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| Abstract: | Transplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor‐β1 (TGF‐β1) induces cardiomyogenic differentiation of adipose‐ derived stromal cells (ADSCs) in vitro. Rat ADSCs were cultured with TGF‐β1 (10 ng ml?1) for 2 weeks in vitro. ADSCs cultured without TGF‐β1 served as a control. The mRNA expression of cardiac‐specific gene was induced by TGF‐β1, while the control culture did not show cardiac‐specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF‐β1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and α‐sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF‐β1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF‐β1 induce ADSC cardiomyogenic differentiation in vitro, which could be useful for myocardial infarction stem cell therapy. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | adult stem cell adipose‐derived stromal cells cardiomyogenic differentiation transforming growth factor‐β 1 osteogenic differentiation |
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