Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat‐denatured malate dehydrogenase |
| |
| Authors: | Rui Nian Duck Sang Kim Lihan Tan Chan‐Wha Kim Woo‐Seok Choe |
| |
| Institution: | 1. Dept. of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 119260;2. The first two authors contributed equally to this work;3. Dept. of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul 136‐710, Korea;4. Dept. of Chemical Engineering, Sungkyunkwan University, Suwon 440‐746, Korea |
| |
| Abstract: | The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone‐mediated refolding of heat‐denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large‐scale refolding processes. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 |
| |
| Keywords: | Malate dehydrogenase (MDH) polyethylene glycol (PEG) molecular bichaperone protein refolding |
|
|
