Cytotoxic Grifolin Derivatives Isolated from the Wild Mushroom Boletus pseudocalopus (Basidiomycetes) |
| |
| Authors: | Junsik Song Md Maniruzzaman Manir Surk‐Sik Moon |
| |
| Institution: | 1. Department of Chemistry, Kongju National University, Kongju 314‐701, Republic of Korea, (phone: +82‐41‐850‐8495;2. fax: +82‐41‐850‐8479) |
| |
| Abstract: | Activity‐guided purification of a MeOH extract of the Korean wild mushroom Boletus pseudocalopus afforded three new grifolin derivatives, 1 – 3 , along with four known phenolic compounds 4 – 7 . Their structures were established by a combination of 1H‐ and 13C‐NMR, NOESY, and extensive two‐dimensional NMR spectroscopic experiments such as gCOSY, gHSQC, gHMBC, and ROESY. The major metabolites 4 and 5 were subjected to reduction to provide the side chain‐reduced compounds 8 and 9 for biological testing. All of the compounds except compound 6 showed anticancer activities in the range of IC50 3.5–11.0 μg/ml against human lung carcinoma A549 and mouse melanoma B16F1 cell lines. In addition, all compounds showed moderate radical‐scavenging activities determined by DPPH assay. |
| |
| Keywords: | Boletus pseudocalopus Grifolin derivatives Cytotoxic activity Fungi |
|
|
