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Direct measurement of the kinetics of CBM9 fusion‐tag bioprocessing using luminescence resonance energy transfer
Authors:Mojgan Kavoosi  A Louise Creagh  Robin F B Turner  Douglas G Kilburn  Charles A Haynes
Institution:1. Dept. of Chemical and Biological Engineering, Michael Smith Laboratories, The University of British Columbia, Vancouver, BC, Canada V6T 1Z4;2. Dept. of Electrical and Computer Engineering, Dept. of Chemistry, Michael Smith Laboratories, The University of British Columbia, Vancouver, BC, Canada V6T 1Z4;3. Dept. of Microbiology and Immunology, Michael Smith Laboratories, The University of British Columbia, Vancouver, BC, Canada, V6T 1Z4
Abstract:
Keywords:affinity chromatography  protein purification  fusion tag  enterokinase  green fluorescent protein (GFP)  linker  luminescence resonance energy transfer (LRET)  high throughput assay  terbium  lanthanide  library
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