Antioxidant Activities of Aqueous Extract from Agrimonia pilosa Ledeb and Its Fractions |
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Authors: | Liancai Zhu Jun Tan Bochu Wang Rui He Yuping Liu Chao Zheng |
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Affiliation: | 1. Bioengineering College, Chongqing University, No.?174, Shapingba Main Street, Chongqing, 400030, P.?R. China (phone: +86‐23‐65112840;2. fax: +86‐23‐65112877);3. Department of Life Science & Chemistry, Chongqing Education College, Chongqing, 400067, P.?R. China;4. Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400030, P.?R. China |
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Abstract: | Agrimonia pilosa Ledeb is used as the tonic for asthenia and fatigue in China. Considering that the energizing effect might be correlated with antioxidant properties, we investigated the antioxidant activities of aqueous extract (AE) from Agrimonia pilosa Ledeb by assessing radical‐scavenging and anti‐lipid‐peroxidation abilities. We found that AE shows a moderate antioxidant activity to scavenge DPPH., O , and .OH and inhibit β‐carotene bleaching with IC50 values of 13.0, 33.2, 351, and 11.9 μg/ml, respectively, while its AcOEt‐soluble fraction (ESF) and BuOH soluble fraction (BSF) exhibit remarkable efficiencies. The ESF's IC50 values of scavenging DPPH., O , and .OH, and inhibiting β‐carotene bleaching are 5.6, 5.8, 171, and 7.6 μg/ml, respectively, and those of BSF are 7.5, 8.4, 82.0, and 6.2 μg/ml, respectively. In addition, we found that there is a significant correlation between total phenol content and the antioxidant activity determined by O and .OH scavenging, and β‐carotene‐bleaching assays. Furthermore, HPLC analysis revealed the presence of quercetin, hyperoside, quercitrin, taxifoliol, luteolin‐7‐O‐β‐D ‐glucopyranoside, and rutin in Agrimonia pilosa Ledeb . Thus, we suggest that the extracts from Agrimonia pilosa Ledeb , could be considered as natural antioxidant sources and dietary nutritional supplements to prevent oxidation‐related diseases. |
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Keywords: | Agrimonia pilosa Antioxidant activity Radical‐scavenging activity Flavonoids Phenols |
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