Hairpin ribozyme catalysis: A surface‐enhanced Raman spectroscopy study |
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Authors: | Aline Percot Sophie Lecomte Jacques Vergne Marie‐Christine Maurel |
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Institution: | 1. Laboratoire de Dynamique, Interactions et Réactivité (LADIR), UMR 7075 CNRS and UPMC Univ Paris 06, 2 rue Henry Dunant, 94320 Thiais, France;2. CBMN, UMR5248 CNRS/Université Bordeaux1, IECB, 2 rue Robert Escarpit 33607 Pessac, France;3. Acides Nucléiques et Biophotonique, FRE 3207 CNRS, Fonctions et Interactions des Acides Nucléiques, UPMC Univ Paris 06, Tour 42, 4 place Jussieu, 75252 Paris Cedex 05, France |
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Abstract: | The existence of an “RNA world” as an early step in the history of life increases the interest for the characterization of these biomolecules. The hairpin ribozyme studied here is a self‐cleaving/ligating motif found in the minus strand of the satellite RNA associated with Tobacco ringspot virus. Surface‐enhanced Raman spectroscopy (SERS) is a powerful tool to study trace amounts of RNA. In controlled conditions, a SERS signal is proportional to the amount of free residues adsorbed on the metal surface. On RNA cleavage, residues are unpaired and free to interact with metal. SERS procedures are used to monitor and quantify the catalysis of ribozyme cleavage at biological concentrations in real time; thus, they propose an interesting alternative to electrophoretic methods. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 384–390, 2009. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com |
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Keywords: | RNA ribozyme hairpin cleavage catalysis surface‐enhanced Raman spectroscopy (SERS) |
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