Comparison of the effects of 1,2-dimethylhydrazine and cyclophosphamide on micronucleus incidence in bone marrow and colon |
| |
Authors: | M T Goldberg D H Blakey W R Bruce |
| |
Affiliation: | Ludwig Institute for Cancer Research, Toronto Branch, 9 Earl Street, Toronto, Ontario M4Y 1M4 Canada |
| |
Abstract: | An in vivo micronucleus assay has been developed that utilizes colonic epithelial cells. The genotoxic effects of 1,2-dimethylhydrazine (54-07-3), a colon carcinogen, and of the nitrogen mustard, cyclophosphamide (50-18-0), on the bone-marrow polychromatic erythrocytes and on colonic epithelium from mice were compared using micronucleus induction in each organ as the end point. In the bone marrow, cyclophosphamide was a potent inducer of micronuclei, while 1,2-dimethylhydrazine administration had little effect on the micronucleus incidence. In the colon, 1,2-diemthylhydrazine was an effective inducer of micronuclei. Thus, the colonic micronucleus assay appears to be a potentially useful test for the detection of colon carcinogens. |
| |
Keywords: | CY cyclophosphamide DMH 1,2-dimethylhydrazine PCE polychromatic erythrocyte |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|