Heat shock response and mammal adaptation to high elevation (hypoxia) |
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Authors: | Xiaolin Wang Cunshuan Xu Xiujie Wang Dongjie Wang Qingshang Wang Baochen Zhang |
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Institution: | (1) Northwest Plateau Biological Research Institute, Chinese Academy of Sciences, Xining, 810001, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China;(3) Affiliated Hospital of Medical College of Qinghai University, Xining, 810001, China;(4) Key Laboratory of Ministry of Science and Technology of China in Henan Normal University, Xinxiang, 453000, China;(5) Key Laboratory of China Ministry of Education in Oncology Research Institute of Western Hospital of Sichuan University, Chengdu, 610041, China;(6) Guangzhou Meizheng Pharmaceutical Industry & Key Laboratory of Ministry of Health of China, Guangzhou, 510075, China;(7) Rubber Research Institute, China Tropical Agricultural Academy of Sciences, Haikou, 571737, China |
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Abstract: | The mammal’s high elevation (hypoxia) adaptation was studied by using the immunological and the molecular biological methods
to understand the significance of Hsp (hypoxia) adaptation in the organic high elevation, through the mammal heat shock response.
(1) From high elevation to low elevation (natural hypoxia): Westem blot and conventional RT-PCR and real-time fluorescence
quota PCR were adopted. Expression difference of heat shock protein of 70 (Hsp70) and natural expression of brain tissue of
Hsp70 gene was determined in the cardiac muscle tissue among the different elevation mammals (yak). (2)From low elevation to high
elevation (hypoxia induction): The mammals (domestic rabbits) from the low elevation were sent directly to the areas with
different high elevations like 2300, 3300 and 5000 m above sea level to be raised for a period of 3 weeks before being slaughtered
and the genetic inductive expression of the brain tissue of Hsp70 was determined with RT-PCR. The result indicated that all of the mammals at different elevations possessed their heat shock
response gene. Hsp70 of the high elevation mammal rose abruptly under stress and might be induced to come into being by high
elevation (hypoxia). The speedy synthesis of Hsp70 in the process of heat shock response is suitable to maintain the cells’
normal physiological functions under stress. The Hsp70 has its threshold value. The altitude of 5000 m above sea level is
the best condition for the heat shock response, and it starts to reduce when the altitude is over 6000 m above sea level.
The Hsp70 production quantity and the cell hypoxia bearing capacity have their direct ratio. |
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Keywords: | heat shock reaction heat shock protein 70(Hsp70) Western blot RT-PCR fluorescence quota PCR high eleva-tion hypoxia mammal stress |
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