Temporal changes in genetic diversity of common bean (Phaseolus vulgaris L.) accessions cultivated between 1800 and 2000 |
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Authors: | M Maras S Sušnik J Šuštar-Vozlič V Meglič |
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Institution: | (1) Agricultural Institute of Slovenia, Ljubljana, SI-1000, Slovenia |
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Abstract: | Fourteen microsatellite markers were used to describe genetic diversity in a sample of 128 common bean (Phaseolus vulgaris L.) accessions cultivated within the territory of Slovenia and its nearby regions between 1800 and 2000. The accessions were
grouped into three periods: period I comprising accessions from the beginning of the 19th century, while the other two periods
included accessions from the middle (period II) and the end of the 20th century (period III). Seven control accessions of
known Mesoamerican and Andean origin were also included in the study. A total of 130 alleles were generated. Allelic richness,
in terms of number of alleles per locus, was 6.07 for period I, 6.71 for period II, and 6.07 for period III. In the UPGMA
dendrogram, all studied accessions were intermixed in three main clusters, indicating that the diversity in the time periods
overlapped. Two clusters consisted of accessions of Andean and Mesoamerican origin, while the third represents additional
variation, which existed in this area already 200 years ago. The analysis of molecular variance showed that a great part of
genetic diversity has been preserved till today, confirming the results of cluster analysis. The calculation of number of
alleles per locus revealed no significant quantitative change in genetic diversity over the last 200 years of common bean
cultivation. However, the calculation of genetic distances indicated slight qualitative shifts in genetic diversity of common
bean germplasm over time, while the calculations of allelic frequency variation and polymorphic information content revealed
recent decline of some alleles’ frequencies. These findings should stress the need for establishing an appropriate strategy
of genetic resources management.
The text was submitted by the authors in English. |
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