Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose |
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| Institution: | 1. Bio-energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea;2. Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China;1. National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;2. University of Chinese Academy of Sciences, Beijing, China |
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| Abstract: | d-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of d-psicose production, thermoactive d-glucose isomerase and the d-psicose 3-epimerase obtained from Bacillus sp. and Ruminococcus sp., respectively, were successfully co-expressed in Escherichia coli BL21 strain. The substrate of one-step enzymatic process was d-glucose. The co-expression system exhibited maximum activity at 65 °C and pH 7.0. Mg2+ could enhance the output of d-psicose by 2.32 fold to 1.6 g/L from 10 g/L of d-glucose. When using high-fructose corn syrup (HFCS) as substrate, 135 g/L d-psicose was produced under optimum conditions. The mass ratio of d-glucose, d-fructose, and d-psicose was almost 3.0:2.7:1.0, when the reaction reached equilibrium after an 8 h incubation time. This co-expression system approaching to produce d-psicose has potential application in food and beverage products, especially softdrinks. |
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| Keywords: | Co-expression High-fructose corn syru p(HFCS) |
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