Bioremediation of pesticide contaminated water using an organophosphate degrading enzyme immobilized on nonwoven polyester textiles |
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| Institution: | 1. Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, Clayton 3168, Australia;2. Textor Technologies, Melbourne, Australia;1. Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi 221005, India;2. Department of Chemical Engineering & Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India;1. Dept. of Structural Biology, The Weizmann Institute of Science, Rehovot, Israel;2. Dept. of Materials and Interfaces, The Weizmann Institute of Science, Rehovot, Israel;3. Dept. of Neurobiology, The Weizmann Institute of Science, Rehovot, Israel;4. The Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany;5. Dept. of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel;1. Faculty of Agriculture and Marine Science, Kochi University, Monobe-Otsu 200, Nankoku, Kochi 7838502, Japan;2. Institute of Lowland and Marine Research, Saga University, Honjo-machi 1, Saga 8408502, Japan;3. National Research Institute of Fisheries and Environment of Inland Sea, Japan Fisheries Research and Education Agency, Maruishi 2–17–5, Hatsukaichi, Hiroshima 7390452, Japan;1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, People’s Republic of China;2. University of the Chinese Academy of Sciences, Beijing 100049, People’s Republic of China |
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| Abstract: | Bioremediation using enzymes has become an attractive approach for removing hazardous chemicals such as organophosphate pesticides from the environment. Enzymes immobilized on solid carriers are particularly suited for such applications. In this study, the organophosphate degrading enzyme A (OpdA) was covalently immobilized on highly porous nonwoven polyester fabrics for organophosphate pesticide degradation. The fabrics were first activated with ethylenediamine to introduce free amine groups, and the enzyme was then attached using the bifunctional crosslinker glutaraldehyde. The immobilization only slightly increased the Km (for methyl parathion, MP), broadened the pH profile such that the enzyme had significant activity at acidic pH, and enhanced the stability of the enzyme. The OpdA-functionalized fabrics could be stored in a phosphate buffer or in the dry state at 4 °C for at least 4 weeks without a large loss of activity. When used in batch mode, the functionalized textiles could degrade 20 μM MP in un-buffered water at liquor to fabric ratios as high as 5000:1 within 2 h, and could be used repeatedly. The fabrics could also be made into columns for continuous pesticide degradation. The columns were able to degrade 50 μM MP at high flow rates, and could be used repeatedly over 2 months. These results demonstrate that OpdA immobilized on nonwoven polyester fabrics is useful in environmental remediation of organophosphate compounds. |
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| Keywords: | Enzyme immobilization Bioremediation Organophosphate Organophosphate hydrolase Polyester Nonwoven textiles |
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