| 遗传修饰小鼠胚胎干细胞种系嵌合体小鼠的研制 |
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| 引用本文： | 程萱,周江,谭晓红,程竞,杨晓.遗传修饰小鼠胚胎干细胞种系嵌合体小鼠的研制[J].生物技术通讯,2003,14(1):1-3. |
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| 作者姓名： | 程萱 周江 谭晓红 程竞 杨晓 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 基金项目： | 国家高技术研究发展计划项目(2001AA216081)，国家自然科学基金项目(30070837和30025028) |
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| 摘 要： | 利用显微注射的方法，分别将三株不同类型的经过遗传修饰的中靶ES细胞注射到C57BL/6J小鼠的囊胚中，通过胚胎移植将注射后的囊胚引入受体小鼠子宫中，分别获得了不同整合度的嵌合体小鼠，将高碳合度小鼠与C57BL/6J小鼠杂交，对这些仔鼠进行PCR及Southern鉴定的结果表明，三株修饰后的ES细胞均能整合入生殖系，得到了棕褐色子代鼠，表明获得了种系嵌合体小鼠。
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| 关 键 词： | 遗传修饰 小鼠 胚胎干细胞 种系嵌合体 显微注射 |
| 文章编号： | 1009-0002(2003)01-0001-03 |
| 修稿时间： | 2002年8月23日 |
Generaion of germ-line chimeras using the genetic modified embryonic stem cells |
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| CHENG Xuan,ZHOU Jiang,TAN Xiao-hong,CHENG Jing,YANG Xiao.Generaion of germ-line chimeras using the genetic modified embryonic stem cells[J].Letters in Biotechnology,2003,14(1):1-3. |
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| Authors: | CHENG Xuan ZHOU Jiang TAN Xiao-hong CHENG Jing YANG Xiao |
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| Abstract: | Three ES cell lines modified by different genetic manipulation were introduced into blastocysts of C57BL/6J mice by microinjection respectively.The injected blastocysts were transplanted into the uterus of pseudopregnant mice.Chimeras with different degrees of the ES cell integration were obtained.Chimeras were crossed with C57BL/6J mice and the offspring carring the modified alleles were identified by PCR and Southern blot hybridization.These results demonstrated that the germ-line transmission mice from three different genetic modified ES cell lines were obtained. |
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| Keywords: | microinjection genetic modification embryonic stem cell germ-line chimera |
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