Axonal growth characteristics of nerve cells of the chick embryo spinal cord in tissue culture

Numerous axons of associated cells were found in the cultures of 13-day old chick embryo spinal cord. These axons formed loops, while leaving the explants, and returned into the cultivated piece. This phenomenon can be due to pronounced specific influence of the explant on the growth of axons of the associated cells via the motoneurons which play the role of target cells.