水稻胚乳特异性启动子Gt1的克隆及其在玉米中的功能验证

以水稻基因组DNA为模板,用PCR方法克隆了水稻谷蛋白基因G t1的启动子序列,并将其构建到带有绿色荧光蛋白基因(gfp)的植物表达载体上,用微束激光穿刺法转化玉米的受体组织,通过外源基因瞬时表达的方法验证了该启动子的功能,结果表明在胚乳中有较强的表达,而在其它组织中表达很弱;证明了水稻谷蛋白基因G t1的启动子在不同物种玉米中同样具有胚乳特异表达的功能。为这一胚乳特异启动子的广泛利用提供了理论依据。

Cloning of a Rice Endosperm-specific Promoter Gt1 and Its Functional Verification in Maize

Regard genome DNA of rice as the template,have cloned the upper sequence of the gluten genetic Gt1 of rice by PCR method,and construct it on the plant expression carriers with green fluorogenic albumen,shell with laser microbeam method receptor to transform maize organize,and start the function of son through the method that express instantly.The result show that it expresses strongly in endosperm,while very weakly in other.It has proved that the peculiar characteristic that expresses of endosperm also expressed in different species too.It offers the theoretical foundation for the use of this endosperm specific promoter.