Fetal bovine serum and other sera used in tissue culture increase epithelial permeability |
| |
Authors: | Kathleen H Mortell Alan D Marmorstein Eva B Cramer |
| |
Institution: | (1) Department of Anatomy and Cell Biology, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, 11203 Brooklyn, New York |
| |
Abstract: | Summary Fetal bovine serum (FBS) or heat-inactivated FBS (56° C for 30 min, HFBS) caused a dose-dependent decrease in the transepithelial
electrical resistance of an epithelial monolayer (MDCK). A saturating concentration of HFBS (30%) caused an average fall of
25 ± 2% within 60 min. Upon removal of HFBS, the resistance returned to its starting value within 1 h. Flux studies with 3H]mannitol demonstrate that the fall in resistance is due to an increased permeability of the tight junctions. Thirty percent
heat inactivated sera from goat, newborn calf, calf, bovine, and horse caused falls ranging from 26 to 47%. In contrast with
the basolateral preference of human and bovine adult sera, fetal bovine and newborn calf sera elicit this response primarily
by interacting with the apical surface of the epithelium. HFBS-treated monolayers show a significant increase in the condensation
of F-actin at points where ≥3 cells meet. These results demonstrate that FBS and other sera used as nutritional supplements
can increase the permeability of the tight junctions of cultured epithelial cells. |
| |
Keywords: | MDCK cells tight junctions actin epithelial permeability factor |
本文献已被 SpringerLink 等数据库收录! |
|