Production of high yields of docosahexaenoic acid byThraustochytrium roseum ATCC 28210

Culture conditions for growth and docosahexaenoic acid (DHA) production byThraustochytrium roseum ATCC 28210 were investigated with a view to increasing DHA titers. A medium was formulated (Medium 6) which produced a biomass and DHA content of 10.4 g L^–1 and 1011 mg L^–1, respectively, in a 5-day incubation. A fed-batch culture system was also developed which achieved biomass and DHA titers of 17.1 g L^–1 and 2000 mg L^–1, respectively, in 12 days.