Steroid transforming enzymes from microorganisms: The reverse reaction of the steroid-1-dehydrogenase from Nocardia |
| |
| Affiliation: | 1. Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab 144411, India;2. Department of Basic and Applied Sciences, National Institute of Food Technology Entrepreneurship and Management, Sonipat 131 028, Haryana, India |
| |
| Abstract: | Hydrogenation and dehydrogenation in position 1(2) of steroids catalyzed during fermentation with Nocardia sp. by only a single enzyme, 3-oxo-4-ene-steroid : (acceptor)-1-ene-oxidoreductase (steroid-1-dehydrogenase) are described. The reversibility of this reaction was confirmed by enzyme induction under aerobic and anaerobic conditions. The mechanism of the hydrogenation reaction was established by H-D-exchange experiments in bisubstrate reactions. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
