The intracellular compartmentation of metabolites in isolated kidney cortex tubules

• 1.1. A method is described to measure cytosolic and mitochondrial metabolites in isolated kidney tubule suspensions.
• 2.2. With the use of digitonin, 95% of cytosolic metabolites are released into the supernatant fraction whereas mitochondrial matrix enzymes are retained in the pellet fraction.
• 3.3. In a study of adaptation to acidosis, different α-oxoglutarate gradients between the tubular cell compartments are obtained, when medium pH is changed from 7.0 to 7.8.