Simultaneous radioimmunoassay of progestins,androgens and estrogens in rat testis |
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| Affiliation: | 1. Normandie Univ, UNICAEN, OeReCa, F-14032, Caen, France;2. INRA, Université de Tours, Centre de recherche de Tours, UMR PRC, Nouzilly, France;3. INRA, Université de Tours, Centre de recherche de Tours, UEPAO, Nouzilly, France;4. Université de Liège, Belgique;5. Normandie Univ, UNICAEN, BIOTARGEN, Caen, France;6. Centre Hospitalier Vétérinaire Équin du Livet, Saint-Michel-de-Livet, France |
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| Abstract: | A reliable method which permits the simultaneous measurement of pregnenolone, progesterone, 17-OH-progesterone, androstenedione, testosterone, dihydrotestosterone, estrone and estradiol in rat testis is described. After extraction of testicular homogenate with methanol, the method includes separation on a LH-20 column and steroid measurement by specific radioimmunoassays. |
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