Analysis of the apparent tissue specific differences in Acheta domesticus ribosomal proteins |
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| Affiliation: | 1. Dept. of Animal and Plant Sciences, The University of Sheffield, S10 2TN, UK;2. Dow AgroSciences, 3 Milton Park, Abingdon, Oxfordshire OX14 4RA, UK;3. Dow AgroSciences, Latchmore Court, Brand Street, Hitchin, Hertfordshire SG5 1NH, UK;1. Department of Food Preservation, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic;2. Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha-Suchdol, Czech Republic;3. Department of Carbohydrates and Cereals, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic;4. Department of Zoology and Fisheries, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha-Suchdol, Czech Republic |
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| Abstract: | One- and two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins isolated from Acheta domesticus male accessory gland, testes and whole body show highly reproducible quantitative and qualitative differences. However, control experiments show that these differences are due either to preferential loss of some ribosomal proteins or to the binding of cell sap proteins during ribosome isolation. No differences could be detected between proteins of 0.25 M and 0.5 M KCl-washed nymph and adult accessory gland ribosomes. The protein composition of ribosomes isolated from various tissues and developmental stages of the cricket appear to be very similar. |
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