Enhancement of human leukocyte elastase activity by ionic strength |
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| Affiliation: | 1. Mechanical Engineering Department, Benha Faculty of Engineering, Benha University, Benha, Egypt;2. Egyptian Space Agency, New Cairo, Egypt;3. Faculty of Engineering and Technology, Badr University in Cairo, Cairo, Egypt;1. Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China;2. Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear water bay, Hong Kong, China |
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| Abstract: | The activity of human leukocyte elastase is strongly enhanced by ionic strength. For instance, with 5 m NaCl, the rate of the elastase catalyzed hydrolysis of N-succinyl-trialanine-p-nitroanilide is increased by a factor of 20. Under these conditions elastase concentrations as low as 1 nm (35 ng/ml) may be assayed accurately. Pancreatic elastase is much less sensitive to ionic strength than the leukocyte enzyme. Between 0.2 and 0.75 m NaCl, the rate of solubilization of elastin by leukocyte elastase is also strongly enhanced. |
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