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Synthesis and DNA-binding ability of Sp1 protein zinc finger domain and its peptidomimetics |
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| Authors: | Rui Wang Jingman Ni Xiaoyu Hu Yaping Ma Xiangqun Li Dingjian Yang Shouliang Dong Xiaowu Yang Xinfu Pan |
| Institution: | 1. Department of Biology, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, 730000, Lanzhou, China 2. Department of Chemistry, Lanzhou University, 730000, Lanzhou, China 3. College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, China |
| Abstract: | The second zinc finger fragment of Sp1 (Sp1-ZF2), its mutant (Sp1-ZF2/HT. E20 → H, R23 → T), and two mimic analogues (ZF20 and ZF15) were synthesized by stepwise solid phase technique. The CD spectra and UV-visible spectrum with CoC12 indicated that the formation of zinc finger structure was affected not only by the hydrophobic amino acids but also by the change of the distance between Cys and His. Gel-retardat ion electrophoresis assays indicated that the Glu and Arg residues are very important for recognition. A single zinc finger like Sp1-ZF2 is able to bind DNA sequence specifically. |
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