Developmental changes in neutral glycosphingolipids of mouse placenta |
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Authors: | Jiri Svejcar Sarah Ehrlich-Rogozinski Dorothea Riedel Johannes Müthing Nathan Sharon |
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Institution: | (1) Institute for Human Genetics, Johann Wolfgang Goethe University, Frankfurt am Main, Germany;(2) Department of Membrane Research and Biophysics, The Weizmann Institute of Science, 76100 Rehovot, Israel;(3) Institute for Cell Culture Technology, University of Bielefeld, Bielefeld, Germany |
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Abstract: | The mammalian placenta is a unique organ for the study of developmental changes. Placentas of laboratory animals such as the mouse allow for the determination of the exact stage of pregnancy, which cannot be achieved with human placenta. In this study, neutral glycosphingolipids were isolated from mouse (inbred strain C57BL/6) placentas, from day 10 to day 18 of gestation, and were separated by high performance thin layer chromatography. Densitometric measurements after orcinol staining showed, at day 10 of gestation, the presence of mono-, tetra-, tri- and dihexosylceramide in decreasing quantities, as well as four unidentified spots. On day 12, the glycosphingolipid composition changed with the disappearance of the unidentified spots and the appearance of an orcinol positive spot migrating similarly to the Forssman antigen; no further changes occurred between days 12 and 18 of gestation. The identity of the Forssman-like glycosphingolipid with the Forssman antigen was established by binding of125I labelledHelix pomatia agglutinin ( -GalNAc specific) to glycosphingolipids separated on high performance thin layer chromatography plates, and by the reaction of the isolated glycosphingolipid with a monoclonal anti-Forssman antibody. The appearance of the Forssman antigen at day 12 of gestation coincided with the day of final maturation of the mouse placenta and subsequent cessation of growth, suggesting a possible role of the glycosphingolipid during embryonic development.Abbreviations asialo-GM1
Gal 3GalNAc 4Gal 4Glc 1Cer
- BCIP
5-bromo-4-chloro-3-indolylphosphate
- DHC
lactosylceramide, Gal 4Glc 1Cer
- Forssman antigen
GalNAc 3GalNAc 3Gal 4Gal 4Glc 1Cer
- globoside
GalNAc 3Gal 4Gal 4Glc 1Cer
- GSL
glycosphingolipids
- HPA
Helix pomatia agglutinin
- HPTLC
high performance thin layer chromatography
- MHC
galactosylceramide, Gal 1Cer
- MHC
glucosylceramide, Glc 1Cer
- PBS
phosphate-buffered saline
- PNA
peanut agglutinin
- PVP
poly(vinylpyrrolidone), mol. wt 40 000
- SBA
soybean agglutinin
- THC
trihexosylceramide, Gal 4Gal 4Glc 1Cer.
To whom correspondence should be addressed. |
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Keywords: | Forssman antigen neutral glycosphingolipids mouse placenta developmental changes |
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