Effect of External Supply of Growth Substances on Axillary Proliferation and Development in Dioscorea bulbifera |
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Authors: | UDUEBO AGNES E |
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Institution: | School of Biological Sciences, University of Lagos Lagos, Nigeria |
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Abstract: | Bulbil development in cultured nodes of D. bulbifera proceededin the absence of growth substances from the medium. When IAAwas incorporated into the medium at the concentrations of 5mg l1 and 10 mg l1 the cultured nodes producedlarger bulbils than in its absences. When the concentrationof IAA was increased to 15 mg l1, however, the culturednodes produced a callus instead of a properly organized bulbil.The dry weight of bulbils increased when kinetin was added tothe medium at the concentrations of 0.05, 0.5, and 2.5 mg l1.The greatest increase was with 0.5 mg l1 kinetin. Onincreasing the concentration of kinetin in the medium to 5.0mg l1 the tissue produced had smaller dry weight thanthose produced in the absence of growth substances. Additionof different combinations of IAA and kinetin to the basal mediumresulted in the production of normal bulbils, roots, and shootsin some instances (suitable combinations) and in the productionof callus and abnormal shoots in others (non suitable combinations). |
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