Comparison of methods to investigate microbial populations in soils under different agricultural management |
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Authors: | Lawlora Knighta Barbosa-Jeffersona Laneb Lilleyc Patond McGratha O'Flahertya Hirscha |
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Institution: | Department of Soil Science, IACR-Rothamsted, Harpenden, Herts AL5 2JQ, UK;Department of Statistics, IACR-Rothamsted, Harpenden, Herts AL5 2JQ, UK;Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, UK;Department of Plant and Soil Science, Cruickshank Building, University of Aberdeen, Aberdeen AB24 3UU, UK |
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Abstract: | As water is a source of nosocomial infections in hospitals, the presence of fastidious Gram-negative bacteria in water samples taken in a university hospital was investigated. Water samples were inoculated onto agar plates and into amoebal microplates for co-culture. Sixty-eight alpha proteobacteria isolates were obtained and characterized using phenotypic methods and 16S rRNA gene sequence comparison. The latter approach divided the strains into seven clusters. Of these, one corresponded to previously recognized Afipia felis and it is likely that six were closely related new species. As these bacteria are fastidious and can not be cultivated on standard microbiological media, their possible role in hospital-acquired human infections should be investigated. |
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Keywords: | Soil microbial population Biolog™ Fatty acid methyl ester Bioluminescence Sewage sludge |
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