Endo-beta-galactosidase of Escherichia freundii. Hydrolysis of pig colonic mucin and milk oligosaccharides by endoglycosidic action. |
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| Authors: | M Fukuda G Matsumura |
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| Affiliation: | Department of Physical Chemistry, Faculty of Pharmaceutical Sciences University of Showa, Shinagawa-ku, Tokyo 145, Japan |
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| Abstract: | The purified keratansulfate degrading enzyme from could hydrolyze desialyzed pig colonic mucin and milk oligosaccharides. Desialyzed pig colonic mucin was digested to produce GlcNAcβ(1→3)Gal, GlcNAc-6Sβ(1→3)Gal and resistant polymer. Lacto-N-tetraose and lacto-N-tetraitol were hydrolyzed endoglycosidically to release glucose and sorbitol, respectively. Therefore, this enzyme was found to be an endo-β-galactosidase of rather wide specificity. |
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| Keywords: | lacto-N-tetraose Galβ(1→3)GlcNAcβ(1→3)Galβ(1→4)Glc lacto-N-tetraitol Galβ(1→3)GlcNAcβ(1→3)Galβ(1→4)Sorbitol GlcNAc-6S N-acetylglucosamine-6-O-sulfate |
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