Physiological relationships between auxin, cytokinin, and a peptide growth factor, phytosulfokine-α, in stimulation of asparagus cell proliferation |
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Authors: | Yoshikatsu Matsubayashi Akiko Morita Emi Matsunaga Akiko Furuya Nobuo Hanai Youji Sakagami |
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Institution: | (1) Laboratory of Bioactive Natural Products Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP;(2) Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Asahi-machi, Machida, Tokyo 194-0023, Japan, JP |
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Abstract: | The aim of this research was to determine whether the production of the mitogenic peptide, phytosulfokine-α (PSK-α), is affected
by auxin and/or cytokinin, and whether the expression of the biological activity of PSK-α requires the presence of these plant
hormones. We developed a competition enzyme-linked immunosorbent assay system that measures the amount of PSK-α using a polyclonal
antibody. In suspension-cultured mesophyll cells of Asparagus officinalis L., the production of PSK-α was first detected after 48 h of culture, prior to the first cell division which was generally
observed after 96 h of culture when both 1-napthaleneacetic acid and N6-benzyladenine were present in the medium. No significant amount of PSK-α was, however, produced when one of these plant hormones
was eliminated from the medium. We also characterized the progression of the cell cycle triggered by PSK-α using a fluorescent
dye and microdensitometry. Asparagus mesophyll cells immediately after isolation were arrested in G0/G1, and the cell cycle proceeded only when all three factors, 1-naphthaleneacetic acid, N6-benzyladenine, and PSK-α, existed in the medium. These results show that the production and the expression of biological
activity of PSK-α is closely correlated with the signal transduction pathway mediated by auxin and cytokinin.
Received: 26 June 1998 / Accepted: 11 November 1998 |
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Keywords: | : Asparagus (cell cycle) Cell cycle Sulfated peptide Phytosulfokine-α |
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