Mannose-binding lectin haplotypes influence Brucella abortus infection in the water buffalo (Bubalus bubalis) |
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Authors: | R Capparelli M Parlato M G Amoroso S Roperto R Marabelli F Roperto D Iannelli |
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Institution: | (1) Faculty of Biotechnological Sciences, University of Naples “Federico II”, Naples, Italy;(2) Faculty of Agriculture, University of Naples “Federico II”, Via Università, 133, 80055 Portici, Naples, Italy;(3) Faculty of Veterinary Medicine, University of Naples “Federico II”, Naples, Italy;(4) Department of Veterinary Public Health, Nutrition, and Food Safety, Ministry of Health, Rome, Italy |
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Abstract: | A case-control study established that the haplotype pair HYA/HYA at the MBL (mannose binding lectin) locus of water buffalo
is associated with resistance to Brucella abortus infection (P < 10−7) and the haplotype pairs LYD/LYD with susceptibility to the same pathogen (P < 10−7). The subjects included in the present study were tested twice—at a 1-month interval—for the presence of anti-B. abortus antibodies in the serum by agglutination, complement fixation and flow cytometry. Cases (335 subjects) included animals consistently
positive to all these tests; controls (335 subjects) comprised animals exposed yet negative by the same tests. The serum from
genetically resistant subjects displayed in vitro significantly higher antibacterial activity compared to the serum from genetically
susceptible subjects, lending biological significance to the results from the association study. Inhibition of the antibacterial
activity following heat treatment of the serum, addition of specific MBL inhibitors (EDTA, mannose, N-acetyl-d-glucosamine) or anti-human MBL antiserum provide convincing evidence that the antibacterial activity present in the serum
results from the interaction between MBL and B. abortus. A replication study (comprising 100 cases and 100 controls) confirmed the results from the original study. |
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Keywords: | Brucella abortus Mannose binding lectin Water buffalo |
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