The Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth |
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Authors: | Roig Patricia Gozalbo Daniel |
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Institution: | Departament de Microbiologia i Ecologia, Facultat de Farmàcia, Universitat de València, Avgda. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. |
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Abstract: | We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Deltaubi3::hisG), previously isolated in the first step of the ura-blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans. |
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Keywords: | UBI3 gene Ribosome biogenesis Gene disruption Conditional null mutant Candida albicans |
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