Amino acid sequences of ovomucoid third domain from 25 additional species of birds |
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| Authors: | Michael Laskowski Jr Izydor Apostol Wojciech Ardelt James Cook Anthony Giletto Clyde A Kelly Wuyuan Lu Soon Jae Park M A Qasim Harry E Whatley Anna Wieczorek and Richard Wynn |
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| Institution: | (1) Department of Chemistry, Purdue University, 47907 West Lafayette, Indiana;(2) Present address: Department of Molecular Biophysics and Biochemistry, Yale University, 06511 New Haven, Connecticut;(3) Present address: Alfacell Corp., 225 Belleville Avenue, 07003 Bloomfield, New Jersey;(4) Present address: Eli Lilly and Company, 307 E. McCarty Street, 46285 Indianapolis, Indiana;(5) Present address: Department of Biochemistry and Molecular Biophysics, Washington University Medical School, 660 S. Euclid, 63110 St. Louis, Missouri;(6) Present address: Biotech Division, Lucky Central Research Institute, Science Town, Dae Jeon, Korea;(7) Present address: Abbott Laboratories, 60064 Abbott Park, Illinois;(8) Present address: Somatogenetics International, Inc., 350 Interlocken Parkway, 80020 Broomfield, Colorado |
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| Abstract: | Ovomucoids were isolated from 25 avian species other than the 101 studied in Laskowskiet al. (1987,Biochemistry
26, 202–221). These were subjected to limited proteolysis with an appropriate enzyme, and connecting peptide extended ovomucoid third domains were isolated and sequenced to the end in a protein sequencer. Of the 25 new sequences, 13 duplicate ones were already known, and 12 are unique. Probably the most striking findings are a Pro14  Ser14 replacement in weka, an Ala14Thr15 replacement in Bulwer's pheasant, the discovery of two additional amino acid residues Ile18 and Gly18 at the P1 reactive site position in Kalij pheasant and tawny frogmouth, respectively, and the first finding of a negative (Glu34) rather than positive (Lys34 or Arg34) amino acid residue at the NH2 terminus of the  helix in caracara ovomucoid third domain. These results complete the determination of all the sequences of ovomucoid third domains in the four species genusGallus, in the five species genusSyrmaticus, and in the two species generaAix andPavo. |
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| Keywords: | Ovomucoid inhibitors of serine proteinases sequences of ovomucoid third domains evolution of birds |
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