Culture of chlorophyllous tobacco-cells not requiring any organic additives except sucrose in the medium I. Effects of light and temperature on the growth of the cells |
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Authors: | Kohno, Hitoshi Yoshida, Fumitake |
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Affiliation: | Laboratory of Soils and Fertilizers, Faculty of Agriculture, Tamagawa University Machida-shi, Tokyo 194, Japan |
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Abstract: | - In the dark, HNG (habituated Nicotiana glutinosa) and NG cellsscarcely grew at 15?C, and the difference between the growthrates of HNG and NG was small at both 15?C and 25?C.
- The stimulatoryeffect of light (4000 lux) on the cell growthrate was higherat 25?C than at 15?C for both HNG and NG.
- Light exerted muchmore effect on the growth rate of HNG thanof NG.
- The thermaleffect was higher in the light than in the darkfor both HNGand NG, and was somewhat greater on NG than onHNG.
- The synergisticeffect of light and temperature on cell growthwas greater onHNG than on NG.
- HNG contained more chlorophyll than NG.
- Inaddition, there was little difference between the friabilitiesof cell groups of HNG and NG.
(Received December 3, 1976; ) |
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