Simple assay of serum copper fractions by ultrafiltration and flameless atomic absorption |
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Authors: | Alain Favier D Ruffieux |
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Institution: | 1. Laboratoire de Biochimie C, Centre Hospitalier Régional de Grenoble, Albert Michallon, BP217 X, 38043, Grenoble Cédex, France
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Abstract: | The authors present a simple and rapid method for assaying ultrafilterable copper (Cu UF) and albumin-bound copper (Cu ALB). It is based on the ultrafiltration of serum in the presence of ethylenediaminetetraacetic acid (EDTA), used to prevent adsorption on the membranes. EDTA at 0.4 g/L has no effect on the equilibrium of serum copper vectors and enables Cu UF to be assayed by flameless atomic absorption. EDTA at 2 g/L, is used to assay total exchangeable copper (CU EXC) (ultrafilterable+albumin-bound). The evaluation criteria of the method are furnished, as are the normal values in healthy subjects: 14.6 μg/L for Cu UF and 87 μg/L for Cu EXC. Finally, the usefulness of the methods described here for the diagnosis of Wilson's and Menkes' disease was demonstrated. |
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