Induction of xylanase inAspergillus ochraceus

Aspergillus ochraceus produced xylanase inductively in washed glucose-grown mycelia incubated with methyl β-d-xyloside. The production of xylan endo-l,4-β-xylosidase (xylanase) was 4.2 times greater than that obtained in xylan medium. The inducer was not metabolized and was most active at a concentration of 0.3 g/L with an incubation period of 16 h. The uptake of the inducer by the mycelia seemed to be energy-dependent. Methyl α-d-xyloside repressed xylanase synthesis. Glucose, cyeloheximide, actinomycin D and eAMP were found to inhibit xylanase induction by methyl β-D-xyloside.