Transfection ofBrevibacterium flavum with bacteriophage BFB10 DNA |
| |
| Authors: | M. Pátek J. NeŠvera J. Hochmannová J. Štokrová |
| |
| Affiliation: | 1. Czechoslovak Academy of Sciences: Deparment of Microbial Genetics, Institute of Microbiology, 142 20, Prague 4
2. Czechoslovak Academy of Sciences: Department of Molecular Virology, Institute of Molecular Genetics, 166 37, Prague 6
|
| |
| Abstract: | The virulent bacteriophageBFB10 is infective toBrevibacterium flavum Its DNA (about 48 kilobase pairs) was used for optimization of the DNA transfer intoB. flavum cells treated with lysozyme. Efficiencies of transfection up to 60 transfectants per ng DNA were obtained The described procedure can also be used for transformation ofB. flavum with plasmid DNA. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
