IV. A model system for mass spectrometric sequencing of orthophthalaldehyde peptide derivatives |
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Authors: | Brent R Larson |
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Institution: | Department of Anatomy University of Arizona Health Sciences Center Tucson, Arizona 85724, USA |
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Abstract: | Presented is a pilot project describing a new strategy for mass spectrometric peptide sequencing. Using orthophthalaldehyde (OPA), two peptides were derivatized and their fluorescent adducts isolated using reversed phase high performance liquid chromatography. The OPA-peptides were permethylated and the derivatized molecules subjected to direct probe mass spectral analysis. The spectra obtained from the OPA-peptides was analogous to those observed for standard -acyl permethyl derivatives enabling the complete sequence of the peptides to be determined. |
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