IgE-mediated 14C-serotonin release from rat mast cells modulated by morphine and endorphins |
| |
Authors: | Yoshihide Yamasaki Osamu Shimamura Akira Kizu Masao Nakagawa Hamao Ijichi |
| |
Affiliation: | 2nd Department of Medicine, Kyoto Prefectural University of Medicine, Kamikyo-ku, Kyoto 602, Japan |
| |
Abstract: | The formaldehyde method was used to examine the interaction of PGE1 with morphine, β-endorphin and Met-enkephalin on rat mast cells by their effects on IgE-mediated 14C-serotonin release. PGE1 (2×10?8?2×10?5 M) caused a dose-related inhibition of the mediator release 1 min after an antigen challenge, and morphine (3×10?7?3×10?5 M) reversed this PGE1 effect dose-dependently and stereospecifically; naloxone (2×10?4 M) antagonized this action of morphine. β-Endorphin (3×10?7?10?5 M) and Met-enkephalin (3×10?6?10?4 M) mimicked this morphine action dose-dependently and were antagonized by naloxone (2×10?4 M). These results suggest that morphine and endorphins modulate immunological mediator release from rat mast cells through opioid receptors. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|