Vitamin E dependent reduced glutathione inhibition of rat liver microsomal lipid peroxidation |
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Authors: | CChanna Reddy Richard W Scholz Craig E Thomas Edward J Massaro |
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Institution: | 1. Center for Air Environment Studies, USA;2. Department of Veterinary Science The Pennsylvania State University University Park, PA 16802, USA |
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Abstract: | Effects of reduced glutathione (GSH) were investigated on lipid peroxidation of hepatic microsomes obtained from Long-Evans Hooded rats fed chemically defined, purified diets containing adequate or documented deficiencies of vitamin E (E), selenium (Se) or both. Glutathione inhibited lipid peroxidation mediated by both NADPH-dependent enzymatic and ascorbate-dependent non-enzymatic systems. The inhibitory effect of GSH was observed in microsomes obtained from E supplemented groups whereas it had no effect on microsomes from E deficient animals. Selenium status had no effect on GSH inhibition. Glutathione was found to be specific for the E dependent inhibition of lipid peroxidation and could not be substituted by other sulfhydryl compounds tested. Also, GSH did not inhibit non-enzymatic lipid peroxidation of heat-denatured microsomes from either E-supplemented groups or any of the other dietary regimens. |
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Keywords: | To whom all correspondence should be addressed |
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