Proteomic identification of altered protein O-GlcNAcylation in a triple transgenic mouse model of Alzheimer's disease |
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Authors: | Antonella Tramutola Nidhi Sharma Eugenio Barone Chiara Lanzillotta Andrea Castellani Federica Iavarone Federica Vincenzoni Massimo Castagnola D Allan Butterfield Silvana Gaetani Tommaso Cassano Marzia Perluigi Fabio Di Domenico |
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Institution: | 1. Department of Biochemical Sciences “A. Rossi Fanelli”, Sapienza University of Rome, Rome, Italy;2. Universidad Autònoma de Chile, Instituto de Ciencias Biomédicas, Facultad de alud, Providencia, Santiago, Chile;3. Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy;4. Department of Chemistry and Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY, USA;5. Department of Physiology and Pharmacology \"V. Erspamer\", Sapienza University of Rome, Rome, Italy;6. Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy |
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Abstract: | PET scan analysis demonstrated the early reduction of cerebral glucose metabolism in Alzheimer disease (AD) patients that can make neurons vulnerable to damage via the alteration of the hexosamine biosynthetic pathway (HBP). Defective HBP leads to flawed protein O-GlcNAcylation coupled, by a mutual inverse relationship, with increased protein phosphorylation on Ser/Thr residues. Altered O-GlcNAcylation of Tau and APP have been reported in AD and is closely related with pathology onset and progression. In addition, type 2 diabetes patients show an altered O-GlcNAcylation/phosphorylation that might represent a link between metabolic defects and AD progression. Our study aimed to decipher the specific protein targets of altered O-GlcNAcylation in brain of 12-month-old 3×Tg-AD mice compared with age-matched non-Tg mice. Hence, we analysed the global O-GlcNAc levels, the levels and activity of OGT and OGA, the enzymes controlling its cycling and protein specific O-GlcNAc levels using a bi-dimensional electrophoresis (2DE) approach. Our data demonstrate the alteration of OGT and OGA activation coupled with the decrease of total O-GlcNAcylation levels. Data from proteomics analysis led to the identification of several proteins with reduced O-GlcNAcylation levels, which belong to key pathways involved in the progression of AD such as neuronal structure, protein degradation and glucose metabolism. In parallel, we analysed the O-GlcNAcylation/phosphorylation ratio of IRS1 and AKT, whose alterations may contribute to insulin resistance and reduced glucose uptake. Our findings may contribute to better understand the role of altered protein O-GlcNAcylation profile in AD, by possibly identifying novel mechanisms of disease progression related to glucose hypometabolism. |
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Keywords: | Alzheimer disease Phosphorylation Glucose metabolism Insulin signaling |
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