MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS |
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| Authors: | Wei Wang Wenbo Tang Erbo Shan Lei Zhang Shiyuan Chen Chaowen Yu Yong Gao |
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| Affiliation: | 1.The First Clinical College of Jinan University, Guangzhou;2.Surgical Oncology Department, the Second Affiliated Hospital of Bengbu Medical College, Bengbu;3.Vascular Surgery Department, the First Affiliated Hospital of Bengbu Medical College, Bengbu;4.General Surgery Department, the Second Affiliated Hospital of Bengbu Medical College, Bengbu, China |
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| Abstract: | MicroRNAs (miRNAs) play critical roles in the development of vascular diseases. However, the effects of miR- 130a-5p and its functional targets on atherosclerosis (AS) are still largely unknown. In this regard, our aim is to explore the potentially important role of miR-130a-5p and its target gene during the progression of endothelial cell injury. We first found oxidized low-density lipoprotein (ox-LDL) induced FAS and cell apoptosis in HUVECs. Subsequently, miR-130a-5p expression was verified to be downregulated after ox-LDL treatment and negatively correlated with FAS, and FAS was identified as substantially upregulated in the ox-LDL-treated HUVEC cells. After that, the knockdown of FAS and overexpression of miR-130a-5p together were observed to aggregate ox-LDL-induced reduction of cell viability and apoptosis, cell cycle progression, cell proliferation, cell migration and invasion. In conclusion, we detected that miR-130a-5p contributed to the progression of endothelial cell injury by regulating of FAS, which may provide a new and promising therapeutic target for AS.Key words: Atherosclerosis, ox-LDL, miR-130a-5p, FAS |
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