| 人乙型肝炎病毒(HBV)adr亚型基因组的多态性 |
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| 引用本文： | 吴祥甫,冯宗铭,江福美,周翊钟,钱斌,李载平. 人乙型肝炎病毒(HBV)adr亚型基因组的多态性[J]. 中国生物化学与分子生物学报, 1985, 1(4): 69-74 |
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| 作者姓名： | 吴祥甫 冯宗铭 江福美 周翊钟 钱斌 李载平 |
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| 作者单位： | 中国科学院上海生物化学研究所(吴祥甫，冯宗铭，江福美，周翊钟，钱斌)，中国科学院上海生物化学研究所(李载平) |
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| 摘 要： | 由乙型肝炎adr亚型病毒(HBVadr)携带者26人的混合血清,得到了HBVadr基因组克隆株(PADR)158株,对这些克隆株进行四种限制性内切酶(BglⅡ,HindⅢ,PstⅠ,XhoⅠ)切点测定,并对其中S株的13种限制性内切酶图谱进行比较研究,发现同为adr亚型病毒,其基因组的限制性酶切图谱存在差异。另外,通过HindⅢ)切点得到的12个克隆株(PADR-H),也进行了酶切图谱分析。在这170个克隆株中,已经发现了5种类型的HBVadr基因组限制性酶切图谱,其中有6种酶(AvaⅠ,EglⅠ,BglⅡ,HincⅡ,HindⅢ,HpaⅠ)的7个变异点。本文报道了HBVadr基因组的多态性现象。
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| 关 键 词： | HBVadr基因 限制性内切酶图谱 多形性 |
| 收稿时间： | 1985-08-20 |
The polymorphism of the genomes from human hepatitis B virus serotype adr |
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| Wu Xiang-fu Feng Zong-ming Jiang Fu-mei Zhou,Yi-zhong Qian,Bin Li,Zai-pingShaughai Institute of Biochemistry,Academia Sinica. The polymorphism of the genomes from human hepatitis B virus serotype adr[J]. Chinese Journal of Biochemistry and Molecular Biology, 1985, 1(4): 69-74 |
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| Authors: | Wu Xiang-fu Feng Zong-ming Jiang Fu-mei Zhou Yi-zhong Qian Bin Li Zai-pingShaughai Institute of Biochemistry Academia Sinica |
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| Abstract: | The cleavage sites of four restriction enzymes (BglⅡ, HindⅢ, Pst Ⅰ , XhoⅠ)in the genomic DNA cloned in the 158 strains from the HBV DNA purified from pooled serum of 26 HBV adr carriers were analyzed. From which 8 strains were further analyzed in detail with 13 restriction enzymes and 4 out of 8 strains different in restriction maps were identified. This results combined the restriction maps of pADR-H, the 12 plasmids of HBV adr DNA cloned through HindⅢ site, showed that there are at least 5 different clones of HBV adr DNA out of the total 170 clones analyzed. Among these 5 strains alteration of 7 restriction sites of 6 restriction enzyme(Ava Ⅰ,Bgl Ⅰ,BglⅡ, HincⅡ, HindⅢ, and Hpa Ⅰ ) has been found. |
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| Keywords: | HBV adr genome Restriction maps Polymorpnism |
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