| 腺相关病毒载体介导siRNA抑制HBV的表达与复制 |
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| 引用本文： | 辛娟娟,孟庆玲,高向东,刘金毅. 腺相关病毒载体介导siRNA抑制HBV的表达与复制[J]. 中国生物工程杂志, 2010, 30(4): 49-53 |
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| 作者姓名： | 辛娟娟 孟庆玲 高向东 刘金毅 |
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| 作者单位： | 1.中国药科大学生命科学与技术学院 南京 210009 2. 中国疾病预防控制中心病毒病预防控制所 北京 1022063.北京三元基因工程有限公司 北京 102600 |
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| 摘 要： | 目的：构建并制备能够有效抑制HBV mRNA表达的siRNA重组腺相关病毒。 方法：将筛选到的siRNA片段克隆入骨架质粒pAAVMCS中，与pAAVRC和pHelper质粒共转染人胚肾293T细胞，包装出重组腺相关病毒，将纯化后的重组病毒直接感染Hep G 2.2.15细胞，通过酶联免疫法和荧光定量PCR法检测重组腺相关病毒的抑制效果。结果：重组腺相关病毒显著降低HBV 分泌的相关抗原蛋白以及DNA和RNA水平。结论：重组腺相关病毒载体介导的siRNA能够有效抑制HBV的表达与复制。
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| 关 键 词： | RNA干扰 HBV 腺相关病毒 基因治疗 |
| 收稿时间： | 2010-02-26 |
Inhibition of Hepatitis B Virus by Small Interfering RNA Expressed from Adenovirus-associated Virus Vectors |
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| XIN Juan-juan,MENG Qing-ling,GAO Xiang-dong,LIU Jin-yi. Inhibition of Hepatitis B Virus by Small Interfering RNA Expressed from Adenovirus-associated Virus Vectors[J]. China Biotechnology, 2010, 30(4): 49-53 |
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| Authors: | XIN Juan-juan MENG Qing-ling GAO Xiang-dong LIU Jin-yi |
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| Affiliation: | 1.School of life Science &; Technology, China Pharmaceutical University, Nanjing 210009,China2.Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China3.Beijing Tri-Prime Genetic Engineering Co. Ltd, Beijing102600, China |
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| Abstract: | Objective:To construct the recombinant Adenovirus-associated virus(AAV) vector for siRNA targeting HBV mRNA and to evaluate the inhibitive effect on Hep G 2.2.15 cell.Method:The packing cell line(human embryonic kidney 293T cell) was co-transfected with AAV backbone plasmid pAAV-MCS which has been cloned into the selected siRNA,along with the pAAV-RC and pHelper in AAV Helper-Free System.The recombinant adenovirus-associated virus was packaged and amplified,followed by infection of the Hep G 2.2.15 cells.Th... |
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| Keywords: | RNA interference HBV Adenovirus-associated virus Gene therapy |
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