A simple method for constructing a tagged protein |
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| Authors: | Jo C Kim J Jo S A |
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| Affiliation: | Department of Biomedical Sciences, National Institute of Health, Seoul [122-701], Korea. |
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| Abstract: | We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day. |
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