Regulation of Azotobacter chroococcum invertase |
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| Authors: | Mercedes G. de la Vega Francisco J. Cejudo Antonio Paneque |
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| Affiliation: | (1) Instituto de Bioquímica Vegetal y Fotosíntesis, Departmento de Bioquímica, Universidad de Sevilla-Consejo Superior de Investigaciones Científicas, Facultad de Biología, Apartado 1113, E-41080 Sevilla, Spain;(2) Present address: Instituto Especial de la Grasa y sus Derivados, CSIC, A partado 1078, E-41080 Sevilla, Spain;(3) Present address: The Sainsbury Laboratory, John Innes Institute, Colney Lane, NR 4 7UH Norwich, UK |
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| Abstract: | Synthesis of the Azotobacter chroococcum invertase was found to be dependent on sucrose or raffinose in the growth medium. The activity of this invertase was slightly inhibited by glucose. Fructose, which by itself did not affect the enzyme activity, protected invertase from glucose inhibition. Per cent residual activity plotted against glucose concentration, and Hill plot indicated that this monosaccharide binds to one interacting site of the enzyme. The results show that regulation of this prokaryotic enzyme clearly differs from that of eukaryotic orgnisms. |
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| Keywords: | Exoenzyme Invertase Azotobacteraceae |
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